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prosieve ex western blot transfer buffer  (Lonza)


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    Lonza prosieve ex western blot transfer buffer
    Prosieve Ex Western Blot Transfer Buffer, supplied by Lonza, used in various techniques. Bioz Stars score: 90/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
    https://www.bioz.com/product/prosieve+ex+western+blot+transfer+buffer/prosieve+ex+western+blot+transfer+buffer/pmc08869955-123-33-40
    Average 90 stars, based on 1 article reviews
    prosieve ex western blot transfer buffer - by Bioz Stars, 2026-09
    90/100 stars

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    Related Articles

    other:

    Article Title: Generation and Characterization of an Inducible Cx43 Overexpression System in Mouse Embryonic Stem Cells
    Article Snippet: Protein samples and Precision Plus Protein WesternC Standards (Bio-Rad) separated by gel electrophoresis (ProSieve EX Running Buffer, Lonza, Basel, Switzerland) were transferred onto a methanol-activated (Carl Roth) 0.22 μm PVDF-FL membrane (Biozym) by tankblot (ProSieve EX Western Blot Transfer Buffer, Lonza).

    Marker:

    Article Title: Proline‑rich polypeptide‑1 decreases cancer stem cell population by targeting BAFF chromatin‑remodeling complexes in human chondrosarcoma JJ012 cells.
    Article Snippet: Polyacrylamide gel electrophoresis and western blotting reagents were supplied by Lonza Group, Ltd., and the experiments were performed according to the manufacturer's protocols. .. The reagents used were as follows: Pager Gold Precast Gels (10% Tris-Glycine; Lonza Group, Ltd.); ProSieve Quad Color Protein marker (4.6-300 kD; Lonza Group, Ltd.); 20X reducing agent for ProSieve ProTrack Dual Color Loading buffer (Lonza Group, Ltd.); ProTrack Loading buffer (Lonza Group, Ltd.); ProSieve ProTrack Dual Color Loading buffer EX running buffer (Lonza Group, Ltd.); ProSieve EX Western Blot Transfer buffer (Lonza Group, Ltd.) and Immobilon®-P Polyvinylidene difluoride membranes (Sigma‐Aldrich; Merck KGaA). .. For the blocking step, Western Blocker solution (Sigma-Aldrich; Merck KGaA) was used.

    Article Title: Epigenetic regulation of embryonic stem cell marker miR302C in human chondrosarcoma as determinant of antiproliferative activity of proline-rich polypeptide 1
    Article Snippet: .. Pager Gold Precast Gels (59502; 10% Tris-Glycine; Lonza, Inc.); ECL reagent (RPN2109; GE Healthcare, Little Chalfont, UK); Western Blocker solution (W0138; Sigma-Aldrich); ProSieve Quad Color Protein marker (4.6-300 kDa, 00193837; Lonza, Inc.); 20× reducing agent for ProSieve ProTrack Dual Color Loading buffer (00193861; Lonza, Inc.); ProTrack Loading buffer (00193861; Lonza, Inc.); ProSieve ProTrack Dual Color Loading buffer EX running buffer (00200307; Lonza, Inc.); ProSieve EX Western Blot Transfer buffer (00200309; Lonza, Inc.); Immobilon ® -P Polyvinylidene difluoride membranes (P4188; Sigma-Aldrich). ..

    Article Title: PRP-1 significantly decreases the ALDH high cancer stem cell population and regulates the aberrant Wnt/β-catenin pathway in human chondrosarcoma JJ012 cells
    Article Snippet: .. Pager Gold Precast Gels (59502; 10% Tris-glycine; Lonza, Inc.); ECL reagent (RPN2109; GE Healthcare, Little Chalfont, UK); Western Blocker solution (W0138; Sigma-Aldrich; Merck KGaA); ProSieve Quad Color Protein marker (4.6–300 kDa, 00193837; Lonza, Inc.); 20X reducing agent for ProSieve ProTrack Dual Color Loading buffer (00193861; Lonza, Inc.); ProTrack loading buffer (00193861; Lonza, Inc.); ProSieve ProTrack Dual Color Loading buffer EX running buffer (00200307; Lonza, Inc.); ProSieve EX Western Blot Transfer buffer (00200309; Lonza, Inc.); Immobilon ® -P polyvinylidene difluoride membranes (P4188; Sigma-Aldrich; Merck KGaA). .. Cytoplasmic and nuclear fractions of cells were isolated following the manufacturer's instructions (cat. no. 40010, lot. no. 22118072; Active Motif) using phosphatase inhibitors, protease cocktail inhibitor and sonicator.

    Article Title: Toll like receptors TLR1/2, TLR6 and MUC5B as binding interaction partners with cytostatic proline rich polypeptide 1 in human chondrosarcoma
    Article Snippet: .. PAGErTM Gold Precast Gels (59502; 10% Tris-Glycine; Lonza); ECL reagent (RPN2109; GE Healthcare, Little Chalfont, UK); Western Blocker solution (W0138; Sigma-Aldrich); ProSieve QuadColor Protein marker (4.6–300 kDa, 00193837; Lonza); 20X Reducing Agent for ProSieve ProTrack Dual Color Loading buffer (00193861; Lonza); ProTrack Loading buffer (00193861; Lonza); ProSieve ProTrack Dual Color Loading buffer EX running buffer (00200307; Lonza); ProSieve EX Western Blot Transfer buffer (00200309; Lonza); Immobilon ® -P PVDF Membranes (P4188; Sigma-Aldrich). .. Rabbit polyclonal anti-TLR6 (ab37072), MW 92 kDa (Abcam); rabbit anti-TLR1 cell (2209), MW 86 kDa (Cell Signaling Technology, Danvers, MA, USA); rabbit anti-TLR1 (ab68158), MW 90 kDa (Abcam); mouse anti-TLR2 [TL2.1], (ab9100), MW 90 kDa (Abcam); Mouse anti-TLR3 (TLR3.7) (sc-32232), MW 104 kDa (Santa Cruz Biotechnology, Santa Cruz, CA, USA); mouse anti-TLR4 ( ) (sc-293072), MW 95-120 kDa (Santa Cruz Biotechnology); mouse anti-TLR5 (19D759.2), (sc-57461), MW 110-120 kDa (Santa Cruz Biotechnology); rabbit anti-TLR7, (5632), MW 140 kDa (Cell Signaling Technology); mouse TLR 8 (9A6), (sc-135584), MW 119.8 kDa (Santa Cruz Biotechnology); rabbit anti-TLR9, (5845), MW 130 kDa (Cell Signaling Technology); mouse TLR10 (2A11), sc-293300, MW 90 kDa (Santa Cruz Biotechnology); mouse anti-tubulin, (T5168; Sigma-Aldrich); rabbit anti-TRIF/TICAM1, NBP2-31189, MW 75 kDa (Novus Biologicals, Littleton, CO, USA); mouse anti-TICAM2, MW 21 kDa (Santa Cruz Biotechnology); rabbit anti-TRAF6 (3566R-100), MW 54 kDa (BioVision, Inc., Milpitas, CA, USA); goat anti-rabbit IgG, HRP-linked (7074; Cell Signaling Technology); anti-mouse IgG, HRP-linked (7076; Cell Signaling Technology).

    Western Blot:

    Article Title: Proline‑rich polypeptide‑1 decreases cancer stem cell population by targeting BAFF chromatin‑remodeling complexes in human chondrosarcoma JJ012 cells.
    Article Snippet: Polyacrylamide gel electrophoresis and western blotting reagents were supplied by Lonza Group, Ltd., and the experiments were performed according to the manufacturer's protocols. .. The reagents used were as follows: Pager Gold Precast Gels (10% Tris-Glycine; Lonza Group, Ltd.); ProSieve Quad Color Protein marker (4.6-300 kD; Lonza Group, Ltd.); 20X reducing agent for ProSieve ProTrack Dual Color Loading buffer (Lonza Group, Ltd.); ProTrack Loading buffer (Lonza Group, Ltd.); ProSieve ProTrack Dual Color Loading buffer EX running buffer (Lonza Group, Ltd.); ProSieve EX Western Blot Transfer buffer (Lonza Group, Ltd.) and Immobilon®-P Polyvinylidene difluoride membranes (Sigma‐Aldrich; Merck KGaA). .. For the blocking step, Western Blocker solution (Sigma-Aldrich; Merck KGaA) was used.

    Article Title: Epigenetic regulation of embryonic stem cell marker miR302C in human chondrosarcoma as determinant of antiproliferative activity of proline-rich polypeptide 1
    Article Snippet: .. Pager Gold Precast Gels (59502; 10% Tris-Glycine; Lonza, Inc.); ECL reagent (RPN2109; GE Healthcare, Little Chalfont, UK); Western Blocker solution (W0138; Sigma-Aldrich); ProSieve Quad Color Protein marker (4.6-300 kDa, 00193837; Lonza, Inc.); 20× reducing agent for ProSieve ProTrack Dual Color Loading buffer (00193861; Lonza, Inc.); ProTrack Loading buffer (00193861; Lonza, Inc.); ProSieve ProTrack Dual Color Loading buffer EX running buffer (00200307; Lonza, Inc.); ProSieve EX Western Blot Transfer buffer (00200309; Lonza, Inc.); Immobilon ® -P Polyvinylidene difluoride membranes (P4188; Sigma-Aldrich). ..

    Article Title: PRP-1 significantly decreases the ALDH high cancer stem cell population and regulates the aberrant Wnt/β-catenin pathway in human chondrosarcoma JJ012 cells
    Article Snippet: .. Pager Gold Precast Gels (59502; 10% Tris-glycine; Lonza, Inc.); ECL reagent (RPN2109; GE Healthcare, Little Chalfont, UK); Western Blocker solution (W0138; Sigma-Aldrich; Merck KGaA); ProSieve Quad Color Protein marker (4.6–300 kDa, 00193837; Lonza, Inc.); 20X reducing agent for ProSieve ProTrack Dual Color Loading buffer (00193861; Lonza, Inc.); ProTrack loading buffer (00193861; Lonza, Inc.); ProSieve ProTrack Dual Color Loading buffer EX running buffer (00200307; Lonza, Inc.); ProSieve EX Western Blot Transfer buffer (00200309; Lonza, Inc.); Immobilon ® -P polyvinylidene difluoride membranes (P4188; Sigma-Aldrich; Merck KGaA). .. Cytoplasmic and nuclear fractions of cells were isolated following the manufacturer's instructions (cat. no. 40010, lot. no. 22118072; Active Motif) using phosphatase inhibitors, protease cocktail inhibitor and sonicator.

    Article Title: Toll like receptors TLR1/2, TLR6 and MUC5B as binding interaction partners with cytostatic proline rich polypeptide 1 in human chondrosarcoma
    Article Snippet: .. PAGErTM Gold Precast Gels (59502; 10% Tris-Glycine; Lonza); ECL reagent (RPN2109; GE Healthcare, Little Chalfont, UK); Western Blocker solution (W0138; Sigma-Aldrich); ProSieve QuadColor Protein marker (4.6–300 kDa, 00193837; Lonza); 20X Reducing Agent for ProSieve ProTrack Dual Color Loading buffer (00193861; Lonza); ProTrack Loading buffer (00193861; Lonza); ProSieve ProTrack Dual Color Loading buffer EX running buffer (00200307; Lonza); ProSieve EX Western Blot Transfer buffer (00200309; Lonza); Immobilon ® -P PVDF Membranes (P4188; Sigma-Aldrich). .. Rabbit polyclonal anti-TLR6 (ab37072), MW 92 kDa (Abcam); rabbit anti-TLR1 cell (2209), MW 86 kDa (Cell Signaling Technology, Danvers, MA, USA); rabbit anti-TLR1 (ab68158), MW 90 kDa (Abcam); mouse anti-TLR2 [TL2.1], (ab9100), MW 90 kDa (Abcam); Mouse anti-TLR3 (TLR3.7) (sc-32232), MW 104 kDa (Santa Cruz Biotechnology, Santa Cruz, CA, USA); mouse anti-TLR4 ( ) (sc-293072), MW 95-120 kDa (Santa Cruz Biotechnology); mouse anti-TLR5 (19D759.2), (sc-57461), MW 110-120 kDa (Santa Cruz Biotechnology); rabbit anti-TLR7, (5632), MW 140 kDa (Cell Signaling Technology); mouse TLR 8 (9A6), (sc-135584), MW 119.8 kDa (Santa Cruz Biotechnology); rabbit anti-TLR9, (5845), MW 130 kDa (Cell Signaling Technology); mouse TLR10 (2A11), sc-293300, MW 90 kDa (Santa Cruz Biotechnology); mouse anti-tubulin, (T5168; Sigma-Aldrich); rabbit anti-TRIF/TICAM1, NBP2-31189, MW 75 kDa (Novus Biologicals, Littleton, CO, USA); mouse anti-TICAM2, MW 21 kDa (Santa Cruz Biotechnology); rabbit anti-TRAF6 (3566R-100), MW 54 kDa (BioVision, Inc., Milpitas, CA, USA); goat anti-rabbit IgG, HRP-linked (7074; Cell Signaling Technology); anti-mouse IgG, HRP-linked (7076; Cell Signaling Technology).



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